Composite

Part:BBa_K4728019

Designed by: Bryant Han   Group: iGEM23_Queens-Canada   (2023-10-10)


TPA transporters expression (TphC, TpiB, TpiA)

Expression of the TPA tripartite transporter proteins (TpiB, TpiA) and the TPA-binding protein (TphC). All three are required to work together for TPA transport.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1509
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1509
    Illegal NotI site found at 3007
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 640
    Illegal BamHI site found at 2356
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1509
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1509
    Illegal NgoMIV site found at 1349
    Illegal NgoMIV site found at 1933
    Illegal NgoMIV site found at 2509
    Illegal AgeI site found at 412
    Illegal AgeI site found at 2747
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None